Time / Day | Mon, 21.2.22 | Tue, 22.2.22 | Wed, 23.2.22 | Thu, 24.2.22 | Fri, 25.2.22 | Mon, 28.2.22 | Tue, 1.3.22 | Wed, 2.3.22 | Thu, 3.3.22 | Fri, 4.3.22 |
Module | Microscopy & Image analysis | Microfluidics | scRNA-seq analysis | |||||||
9:00 | Introduction to light microscopy | Homework Discussion | Homework Discussion | Scrum meeting | Introduction into microfluidics | Student presentations | ||||
Image processing I: Filters, thresholding, segmentation | Image processing II: Machine learning, tracking | Scientific lecture II | Introduction to scRNA-seq | Homework discussion and exercises in ‘R’ | Scientific lecture IV (Antonio Scialdone) | Scientific lecture V | ||||
10:00 | ||||||||||
Confocal acquisition (interactive demo) | Fiji III: Segmentation Fiji IV: Automation | Fiji V: ML tools | Coffee break | Coffee break | Coffee break | Coffee break | ||||
Getting started with scRNA-seq data analysis | Machine learning tools I | Homework discussion and scRNA-seq data from pre-implan- tation mouse embryos I | Homework discussion and scRNA-seq data from gastru- lating mouse embryos I | |||||||
11:00 | Q’n’A Break | How to run microfluidics experiments | ||||||||
Project work II | ||||||||||
Epifluorescence acquisition (interactive demo) | Fiji II: ROI manager, measurements | |||||||||
12:00 | Project outline | Lunch break | Lunch break | Lunch break | Lunch break | |||||
Quiz I | Quiz II | |||||||||
Lunch break | Lunch break | Lunch break | Lunch break | Lunch break | Lunch break | |||||
13:00 | Some machine learning tools Homework assignment | Machine learning tools II Homework assignment | scRNA-seq data from pre-implan- tation mouse embryos II Homework assignment | scRNA-seq data from gastru- lating mouse embryos II Concluding remarks | ||||||
Fiji I: Image I/O, basic manipulations | Fiji and R. Model fitting, plotting, statistics | Project work I | Scrum meeting | Single cell analysis in Matlab | Scientific lecture III | |||||
Project work III | ||||||||||
14:00 | ||||||||||
15:00 | Scientific lecture I | |||||||||
Home- work | Homework I | Homework II | Project work | Project work | Prepare presentations | Homework | Homework | Homework |